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microfluidic polydimethylsiloxane (pdms) co-flow devices drop-seq chips  (FlowJEM Inc)

 
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    FlowJEM Inc microfluidic polydimethylsiloxane (pdms) co-flow devices drop-seq chips
    Cell preparation for droplet-based single-cell transcriptional profiling. Schematic of experimental workflow. Cultured human ( HEK ) and mouse ( 3T3 ) cells were dissociated, mixed and further processed to analyse the transcriptomes of either live or fixed cells by Drop-seq. Washed cells were gently resuspended in 2 volumes of ice-cold PBS, then fixed by adding 8 volumes of ice-cold methanol. Methanol-fixed cells could be stored for up to several weeks at –80 °C. Prior to Drop-seq, cells were washed before passing them through a 35- to 40-μm cell strainer. Cells were then separately encapsulated in droplets together with a single bead in a <t>microfluidic</t> co-flow device and single-cell transcriptomes sequenced in a highly parallel manner. Downstream analysis and systematic quantitative comparisons were subsequently made from separate experiments using live or fixed cellular input material with an R package ('dropbead') that we developed and is freely available for download
    Microfluidic Polydimethylsiloxane (Pdms) Co Flow Devices Drop Seq Chips, supplied by FlowJEM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microfluidic+polydimethylsiloxane+(pdms)+co-flow+devices+drop-seq+chips/drop+seq+microfluidic+device/pmc05438562-79-11-18
    Average 90 stars, based on 1 article reviews
    microfluidic polydimethylsiloxane (pdms) co-flow devices drop-seq chips - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Cell fixation and preservation for droplet-based single-cell transcriptomics"

    Article Title: Cell fixation and preservation for droplet-based single-cell transcriptomics

    Journal: BMC Biology

    doi: 10.1186/s12915-017-0383-5

    Cell preparation for droplet-based single-cell transcriptional profiling. Schematic of experimental workflow. Cultured human ( HEK ) and mouse ( 3T3 ) cells were dissociated, mixed and further processed to analyse the transcriptomes of either live or fixed cells by Drop-seq. Washed cells were gently resuspended in 2 volumes of ice-cold PBS, then fixed by adding 8 volumes of ice-cold methanol. Methanol-fixed cells could be stored for up to several weeks at –80 °C. Prior to Drop-seq, cells were washed before passing them through a 35- to 40-μm cell strainer. Cells were then separately encapsulated in droplets together with a single bead in a microfluidic co-flow device and single-cell transcriptomes sequenced in a highly parallel manner. Downstream analysis and systematic quantitative comparisons were subsequently made from separate experiments using live or fixed cellular input material with an R package ('dropbead') that we developed and is freely available for download
    Figure Legend Snippet: Cell preparation for droplet-based single-cell transcriptional profiling. Schematic of experimental workflow. Cultured human ( HEK ) and mouse ( 3T3 ) cells were dissociated, mixed and further processed to analyse the transcriptomes of either live or fixed cells by Drop-seq. Washed cells were gently resuspended in 2 volumes of ice-cold PBS, then fixed by adding 8 volumes of ice-cold methanol. Methanol-fixed cells could be stored for up to several weeks at –80 °C. Prior to Drop-seq, cells were washed before passing them through a 35- to 40-μm cell strainer. Cells were then separately encapsulated in droplets together with a single bead in a microfluidic co-flow device and single-cell transcriptomes sequenced in a highly parallel manner. Downstream analysis and systematic quantitative comparisons were subsequently made from separate experiments using live or fixed cellular input material with an R package ('dropbead') that we developed and is freely available for download

    Techniques Used: Cell Culture

    Related Articles

    Generated:

    Article Title: Cell fixation and preservation for droplet-based single-cell transcriptomics
    Article Snippet: Monodisperse droplets of about 1 nl in size were generated using microfluidic polydimethylsiloxane (PDMS) co-flow devices (Drop-seq chips, FlowJEM, Toronto, Canada; rendered hydrophobic by pre-treatment with Aquapel).

    Cell Culture:

    Article Title: Cell fixation and preservation for droplet-based single-cell transcriptomics
    Article Snippet: Monodisperse droplets of about 1 nl in size were generated using microfluidic polydimethylsiloxane (PDMS) co-flow devices (Drop-seq chips, FlowJEM, Toronto, Canada; rendered hydrophobic by pre-treatment with Aquapel).



    Similar Products

    microfluidic polydimethylsiloxane (pdms) co-flow devices drop-seq chips  (FlowJEM Inc)
    90
    FlowJEM Inc microfluidic polydimethylsiloxane (pdms) co-flow devices drop-seq chips
    Cell preparation for droplet-based single-cell transcriptional profiling. Schematic of experimental workflow. Cultured human ( HEK ) and mouse ( 3T3 ) cells were dissociated, mixed and further processed to analyse the transcriptomes of either live or fixed cells by Drop-seq. Washed cells were gently resuspended in 2 volumes of ice-cold PBS, then fixed by adding 8 volumes of ice-cold methanol. Methanol-fixed cells could be stored for up to several weeks at –80 °C. Prior to Drop-seq, cells were washed before passing them through a 35- to 40-μm cell strainer. Cells were then separately encapsulated in droplets together with a single bead in a <t>microfluidic</t> co-flow device and single-cell transcriptomes sequenced in a highly parallel manner. Downstream analysis and systematic quantitative comparisons were subsequently made from separate experiments using live or fixed cellular input material with an R package ('dropbead') that we developed and is freely available for download
    Microfluidic Polydimethylsiloxane (Pdms) Co Flow Devices Drop Seq Chips, supplied by FlowJEM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microfluidic+polydimethylsiloxane+(pdms)+co-flow+devices+drop-seq+chips/drop+seq+microfluidic+device/pmc05438562-79-11-18
    Average 90 stars, based on 1 article reviews
    microfluidic polydimethylsiloxane (pdms) co-flow devices drop-seq chips - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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    Cell preparation for droplet-based single-cell transcriptional profiling. Schematic of experimental workflow. Cultured human ( HEK ) and mouse ( 3T3 ) cells were dissociated, mixed and further processed to analyse the transcriptomes of either live or fixed cells by Drop-seq. Washed cells were gently resuspended in 2 volumes of ice-cold PBS, then fixed by adding 8 volumes of ice-cold methanol. Methanol-fixed cells could be stored for up to several weeks at –80 °C. Prior to Drop-seq, cells were washed before passing them through a 35- to 40-μm cell strainer. Cells were then separately encapsulated in droplets together with a single bead in a microfluidic co-flow device and single-cell transcriptomes sequenced in a highly parallel manner. Downstream analysis and systematic quantitative comparisons were subsequently made from separate experiments using live or fixed cellular input material with an R package ('dropbead') that we developed and is freely available for download

    Journal: BMC Biology

    Article Title: Cell fixation and preservation for droplet-based single-cell transcriptomics

    doi: 10.1186/s12915-017-0383-5

    Figure Lengend Snippet: Cell preparation for droplet-based single-cell transcriptional profiling. Schematic of experimental workflow. Cultured human ( HEK ) and mouse ( 3T3 ) cells were dissociated, mixed and further processed to analyse the transcriptomes of either live or fixed cells by Drop-seq. Washed cells were gently resuspended in 2 volumes of ice-cold PBS, then fixed by adding 8 volumes of ice-cold methanol. Methanol-fixed cells could be stored for up to several weeks at –80 °C. Prior to Drop-seq, cells were washed before passing them through a 35- to 40-μm cell strainer. Cells were then separately encapsulated in droplets together with a single bead in a microfluidic co-flow device and single-cell transcriptomes sequenced in a highly parallel manner. Downstream analysis and systematic quantitative comparisons were subsequently made from separate experiments using live or fixed cellular input material with an R package ('dropbead') that we developed and is freely available for download

    Article Snippet: Monodisperse droplets of about 1 nl in size were generated using microfluidic polydimethylsiloxane (PDMS) co-flow devices (Drop-seq chips, FlowJEM, Toronto, Canada; rendered hydrophobic by pre-treatment with Aquapel).

    Techniques: Cell Culture